FLOW MICROFLUORIMETRY Meaning and
Definition
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Flow microfluorimetry is a specialized technique used in the field of biotechnology and molecular biology to quantitatively measure the fluorescence emitted from individual cells or particles in a flowing fluid. It involves the analysis of fluorescence intensity of labeled biological samples, typically using a flow cytometer.
Flow cytometry is a powerful method that allows the simultaneous analysis of multiple parameters of individual cells or particles in suspension. In flow microfluorimetry, the flow cytometer utilizes laser light to excite fluorochromes or fluorescent dyes that are bound to specific targets within the biological sample. The emitted fluorescence from each labeled particle is then measured by photomultiplier tubes and converted into electrical signals for subsequent data analysis.
This technique offers several advantages, including high sensitivity, rapid analysis, and the ability to investigate large populations of cells or particles. Flow microfluorimetry enables the quantification of different parameters such as cell size, morphology, complexity, and the presence or absence of specific biomarkers.
The applications of flow microfluorimetry are diverse and include research in immunology, cancer biology, microbiology, and drug discovery. It can be used to study the immune response to infectious agents, analyze cell cycle progression, examine cell viability, and assess the effectiveness of therapeutics. Overall, flow microfluorimetry serves as an essential tool for scientists to gain insights into cellular behavior, disease mechanisms, and therapeutic responses at a single-cell level.
Common Misspellings for FLOW MICROFLUORIMETRY
- dlow microfluorimetry
- clow microfluorimetry
- vlow microfluorimetry
- glow microfluorimetry
- tlow microfluorimetry
- rlow microfluorimetry
- fkow microfluorimetry
- fpow microfluorimetry
- foow microfluorimetry
- fliw microfluorimetry
- flkw microfluorimetry
- fllw microfluorimetry
- flpw microfluorimetry
- fl0w microfluorimetry
- fl9w microfluorimetry
- floq microfluorimetry
- floa microfluorimetry
- flos microfluorimetry
- floe microfluorimetry
- flo3 microfluorimetry
Etymology of FLOW MICROFLUORIMETRY
The word "Flow Microfluorimetry" is derived from multiple roots:1. Flow: The term "flow" refers to the movement or transfer of a substance in a continuous, smooth manner. In the context of flow cytometry, it specifically indicates the process of cells or particles passing through a fluidic system in a controlled way.2. Micro-: This prefix is derived from the Greek word "mikros", meaning small. It is used to denote something on a microscopic or very small scale.3. Fluorimetry: This term is a combination of "fluorescence" and "spectrometry". Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation, while spectrometry refers to the measurement of the properties of light, such as intensity or wavelength. Combining these two terms, "fluorimetry" indicates the measurement of fluorescence emitted by a substance.
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